CONVENTIONAL PRODUCTION OPTIMIZATION OF CYCLODEXTRIN GLUCOSYL TRANSFERASE BY A NOVEL ISOLATE OF BACILLUS SP. PBS1 FROM POTATO RHIZOSPHERE

The cyclodextrin glucosyltransferase enzyme (CGTase) is an industrially crucial for the production of β-cyclodextrin (β-CD). CGTase has a high propensity to produce mixture cyclodextrins (CDs). From industrial perspective, that produces only one type CD critical importance. Bacillus sp. PBS1 produced converts starch solely into β-CD. isolated strain was found close similarity with alkaliphilic based on biochemical, morphological, and phylogenetic analysis its 16s rRNA gene sequencing. selection optimization media ingredients are warranted best possible These steps were carried out by conventional strategies. presence glucose, maltose, lactose, sucrose, galactose, mannitol, nitrates, urea, metal salts, K2HPO4 led suppression production. improved observed in peptone, soluble starch, magnesium sulfate, Na2CO3. organism maximum (93.42 ± 2.4 U/ml) at 96-hour incubation optimized medium containing 8% 2% 0.06% MgSO4.7H2O, 0.5% Na2CO3, having pH 9.3. ~16% improvement PBS1.